سه شنبه 89/9/23  ساعت 3:45 عصر

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Interactions of sodium selenat and sodium selenite with DNA

Maryam montazeri, F. Manuchehri, N. Makouie,

*A. Panahyab, Sh. Nafisi

Department of Chemistry, Islamic Azad University, Central Tehran

Branch (IAUCTB), Tehran, Iran

Selenium has long been of interest for possible cancer chemo preventive effects, largely assumed to be due to its role in the antioxidant enzyme glutathione peroxidase. Recent studies suggest that selenium compounds may increase oxidative stress as well as relieve it. Sodium selenite has been found to be an effective prophylactic treatment for erysipelas (a Streptococcal infection of the skin) in patients with secondary lymphoedema after cancer surgery. The anticancer and antiviral effects of these natural products are attributed to their potential biomedical applications. Sodium selenate and sodium selenite bindings to DNA and RNA are not fully investigated. This study was designed to examine the interactions of one of the most important kinds of antioxidants, called sodium selenate and sodium selenite with calf-thymus DNA in aqueous solution at physiological conditions, using constant DNA concentration (0.51 mM) and various concentration of drudge. sodium selenate and sodium selenite can stimulate the central nervous system by inhibiting the metabolisms of amine neurotransmitters or by direct interaction with specific receptor. UVvisible spectroscopic methods were used to determine the ligand binding modes, the binding constant and the stability of sodium selenate and sodium selenite-DNA complexes in aqueous solution. Spectroscopic showed major binding of sodium selenate and sodium selenite to DNA with overall binding constants of

K sodium selenate = 5.2 x 104 M-1, where K is the binding constant.

K sodium selenite = 1.5x 103 M-1, where K is the binding constant

Key words: DNA, sodium selenate, sodium selenite, UV-visible spectroscopic.

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